Sep 15, 2019 FCSalyzer is a free program for analysing flow cytometry data. It is programmed in Java and should therefore work on many different operating systems. FCSalyzer offers an easy What-you-see-is-what-you get interface and provides the standard analysing tools - dot plots, histograms, complex gating strategies and associated statistics. Flow Cytometry Applications. Benchtop flow cytometry from Luminex offers rapid access to insightful cellular analysis that you can apply to diverse research areas, including immunology and molecular biology. Because the systems are easy to use and affordable to. CytExpert is a highly capable software program that controls instrument operation, and data collection and analysis. Novice to experienced flow cytometrists can learn to operate the system quickly, confidently set up experiment based protocols and export publication quality data. Default installation option requires no user login.
Fast, accurate, automatic analysis
Trained on thousands of samples, ModFit LT features a robust analysis engine. Epson perfection 4990 scan software mac. The sophisticated peak detection system identifies ploidy patterns and dynamically creates models to match. AutoDebris™, AutoAggregate™, and AutoLinearity™ make analysis robust and consistent. No other cell cycle analysis software comes close!
Ploidy determinations are no longer bound to the peak order. Make use of internal standards or an external reference to determine which cycle is classified as Diploid. Any number of cell cycles can be analyzed.
From letterhead graphics to text, ModFit LT imports from the clipboard everything you need to create your own reusable report templates. Save your custom reports with all the text formatting, analysis information, and even the histogram data.
Database and graphic output
ModFit LT allows you to automatically add to a database each time you analyze a data file. Select the items you want to database from an extensive list of results and keywords supported in the sample. Output can be easily opened by programs like Microsoft Excel and your LIS.
Process groups of data files with ease using ModFit LT. The batch system automatically loads data, analyzes, and saves reports. Plus, you can batch process reports for easy review of analysis and printing.
Short learning curve
Simple design and operation make ModFit LT easy to learn and use. We have even created video tutorials to help you get going as quickly as possible.
Download a free version of ModFit LT Rule-Based Training System with your purchase.
Click here for the latest features and improvements in ModFit LT.
Automatic and manual analysis
Reading Flow Cytometry Data
While auto analysis is used for the majority of histograms, you can take control of scaling, model selection, and range positioning for manual analysis. A Quick Model Editor makes it easy to make adjustments to the model.
Your system can be custom configured for linearity, S-phase modeling, number of standards, and model colors used by the program. In addition, peak-finding adjustments can fine-tune the program to the subtleties of your data.
Create high-resolution, 2-parameter gates to select the population to be analyzed. Freehand and polygonal regions make gating quick and efficient, yet each can be modified with the click of a mouse. Gate graphics appear on the report page automatically, making live gate adjustments a snap.
Windows versions support Dynamic Data Exchange (DDE), allowing users to create custom extensions and automation solutions for cytometry analysis.
Models built from Gaussian, rectangle, trapezoid, polynomial, AutoDebris™, and AutoAggregate™ components are processed by the Marquardt nonlinear least-squares analysis. The models used in ModFit LT's cytometric analysis have been tested with thousands of histograms to ensure accurate results.
Quantitative S-phase assessment system
Based on a statistical method for determining the standard deviation for the S-phase estimate, ModFit LT provides a p-value for classifying your S-phase result within your laboratory cutoffs.
S-phase cutoff values
Enter your S-phase cutoff values for any type of tissue you analyze. Separate cutoffs are provided for DNA Diploid, DNA Aneuploid, and Total (Average) S-phase percentages.
Flow Cytometry Analysis In R
Synchronization and Cell-Tracking wizards
Flow Cytometry Graph Analysis
Analyze synchronized cell lines and other perturbed samples with powerful models created by a simple-yet-sophisticated wizard. Choose from options in the wizard dialog to match the model to your data, and let the wizard do the rest. You can even analyze cell-tracking dye samples like CSFE with the Cell-tracking wizard.